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Simultaneously, BCAA-mediated mTORC1 hyperactivation in podocytes suppresses autophagy and exacerbates insulin resistance, creating a metabolic milieu that potentiates toxin-induced injury (136, 149)

The culture medium was exchanged by an extracellular solution containing (in mM) 140 NaCl, 2.5 KC1, 1 MgCl 2 , 3 CaCl 2 , 25 glucose, and 5 HEPES

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