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10.1038/nature13909 28 CoxF

Genomic DNA was then isolated with the help of NucleoSpin Tissue XS from the clonal cell lines and subjected to sequencing to identify frameshift mutations by amplifying the genomic regions with the following primers: for ARL8A KO, forward 5- GATTCCGGGGCAGCGAGTCGT-3 and reverse 5-TACTAGGCCCCAAGCGCTCG-3, and for ARL8B KO, forward 5-TCATCTCCCGCCTGCTGGACT-3 and reverse 5-TCCAAATGCTGGCCCTCGG-3

This demonstrates that whole-body glucose homeostasis is tightly linked to insulin-mediated control of glucose transport and metabolism in adipocytes

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Exercise, physical activity, sun, heat, airline travel, and alcohol can worsen swelling