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Transcriptome profiling of in vitro -matured oocytes from a Korean native cow (hanwoo) after cysteamine supplementation

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To the 10.000 rpm supernatant containing microsomes and cytosol fractions, 0.2 volume of 0.1 M calcium chloride solution was added, and further centrifuged at 10,000 rpm for 10 minutes to precipitate the microsomes

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Alternate nostrils with each spray

Results Sperm motility increased 2-3 months after perceived stress As our prior studies identified the timing at which prior perceived stress score (PSS) in men was associated with significant changes in sperm small non-coding RNA (sncRNA) content important for fertility 50 , we hypothesized that prior perceived stress at critical time points may also increase sperm motility in a human cohort