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cAMP accumulation HEK 293T cells were cultured in Dulbeccos Modified Eagles Medium (DMEM) (Gibco, Waltham, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco) and 1% (v/v) sodium pyruvate (Gibco) at 37 C, 5% CO 2 in a humidified incubator