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Shortcomings of current models of glucose-induced insulin secretion

Spiking experiments into the plasma fraction prior to extraction showed that enzymes catalyzing the conversion of NADH to NAD+ are localized in plasma, while those affecting NAD + , NADP + and NADPH are associated with the cellular fraction (Supplementary Table 1, Sample Type 4)

All components were mixed, and reactions were performed in 100 l volume in 96-well microtitre plates (PS, F-Bottom, clear, Greiner)

Plain glutathione delivered to the plasma by precursors, liposomal products or intravenously must be broken down by enzymes to the basic amino acid components for absorption into the cell and require more energy expenditure to be re-constructed back to rGSH