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Jonathan D Douros provided the compound, and offered scientific guidance on the project

As for free testosterone, the studies by La Vignera et al

Cells were seeded in 96-well plates (3,000 cells per well) 1 day before treatment

Antioxidants are molecules that neutralize harmful oxygen-derived free radicals, protecting skin cells from damage and helping to maintain healthy cell membranes

Samples were prepared in triplicate and were stored in the dark at 42 C with 25% relative humidity (RH) in an accelerated degradation chamber, as 2 weeks storage under these conditions is equivalent to approximately 6 weeks storage under ambient conditions [29, 30]