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Throughout the experimental period, animals were monitored daily for signs of distress, including severe lethargy, impaired mobility, abnormal posture, or inability to access food and water

3.3 Risk of bias of included studies The results of the risk of bias assessment of the included articles using the RoB-2 tool are presented in Figures 3 , 4

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H (400 MHz, CDCl 3 ): 8.79 (t, 2H J = 5.2 Hz, NH), 3.603.53 (m, 4H, CH 2 ), 3.33 (s, 4H, CH 2 ), 3.15 (q, 4H, J = 5.8 Hz, CH 2 ), 1.661.56 (m, 12H)