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Our roGFP2 measurements confirm (i) that dynamic roGFP2 sensing (or the physiological glutathionylation/deglutathionylation of regular surface-exposed cysteinyl residues) requires a glutaredoxin activity, and (ii) that glutathionylated proteins or metabolites should be very transient redox species when GSH and glutaredoxins are present (unless they are stabilized by a kinetic uncoupling mechanism) 5,32,34,35,61,62,63,64

This magnitude of ascorbate degradation is less than the approximate 90% loss described for broccoli stored at 20C ( Figure 6 Figure 7 On day 0 of storage, a 40% greater concentration of GSH was present in the florets of the 20 M EGT-treated broccoli relative to the control ( Figure 7 )