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Lastly, it is notable that the liquid medium routinely used for induction of gliotoxin biosynthesis (i.e., Czapek-Dox) provides a zinc-limiting culture condition (Saleh et al., 2018), which strongly suggests that zinc starvation could be the primary stimulus that triggers the ZafA-mediated induction of gliZ expression and, hence, gliotoxin biosynthesis, whereas the function of GliZ would be dedicated to maintain steady expression levels of the gli genes and gliotoxin biosynthesis while zinc starvation persists

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