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If using a powder product that requires reconstitution, prepare only what will be used within a reasonable timeframe

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After incubation, formic acid (at a final concentration of 1%) was added to the mixture, which was centrifuged at 14000 g for 5 min

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For the analysis of the urine samples, hydrophilic interaction liquid chromatography-high resolution high accuracy mass spectrometry (HILIC-HR-MS) was employed [7]