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If your draw is less than about 1 unit, the volume is too small to read accurately add more bacteriostatic water next time

The orchestration of mouse locomotor behavior involves various brain regions such as the motor cortex, basal ganglia, thalamus, mesencephalic locomotor region (MLR), brainstem (pons/medulla), and cerebellum 82,83

American Journal of Preventive Medicine , 42 (5), e57e64

The procedure is carefully controlled for temperature and timing, with incubations typically ranging from 1 to 2 h at room temperature to overnight settings at 4C, depending on the target analyte

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In vivo and in vitro models of PD demonstrated that exendin-4 ameliorated behavioral deficits and reduced -synuclein pathology, and enhanced autophagy via restoration of PI3K/Akt/mammalian target of rapamycin (mTOR) signaling [84]