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To induce cellular quiescence, the cells were serum deprived for 24 h prior to initiating the treatments

Physical dependence may not occur to a clinically significant degree until after several days to weeks of continued use

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This is risky for several reasons: Wrong patient selection, such as people with pancreatitis, gallstones, or severe kidney disease using them without screening Rapid, unsupervised dose escalation No monitoring of kidney, liver, pancreatic, or eye status Complications seen with unsupervised use include pancreatitis, dehydration-induced acute kidney injury (especially in hot Indian summers with vomiting or diarrhoea), and even diabetic ketoacidosis in susceptible individuals

Clinical data across hundreds of participants shows serious adverse events are essentially nonexistent with topical use

Each approach should be tailored to individual needs and medical guidance