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Fragments were PCR amplifed and gel extracted with a Zymo gel extraction kit before being assembled into pMXS-puro linearized with BamHI and NotI with a 4-component Gibson reaction

No Phase 1, Phase 2, or observational human study exists at the time of writing

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doi: 10.1177/1753425913501098 87 SongNFangYSunXJiangQSongCChenMet al