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10.7554/eLife.33221 eLife 124 WilsonR

Cell Cultures Human papillary thyroid carcinoma cell lines, like TPC-1 and KTC-1 with STR identification, were cultured in 1640 (Gibco, Detroit, MI, USA) in a humid atmosphere of 95% air and 5% CO 2 at 37C, which was routinely supplemented with 10% fetal bovine serum (Gibco, Detroit, MI, USA), penicillin (100 units/ml, Grand Island, NY, USA), and streptomycin (100 g/ml, Grand Island, NY, USA)

HNMT, by contrast, is found in almost all organs in the body, including in the brain and red blood cells

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Its been linked to hormonal changes and even sexual dysfunction in some users (Mysore, 2012)

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