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The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm

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CVS is likely common, being present in about 2% of Scottish [2] and Western Australian [3] school children

On the fourth day, 1 mg ml of l-(4, 5-Dimethylthiazol-2-yl) 3, 5-diphenyIformazan reagent (MTT, Sigma- Aldrich) prepared in PBS was added to the cells and incubated for 4 h

Biochemia Medica 25(2):141151 Guo X, Yu Y, Liu X, Zhang Y, Guan T, Xie G, Wei J (2014) Heterologous expression and characterization of human cellular glutathione peroxidase mutants