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The possible explanation for the increased spontaneous acrosome reaction as seen in this study could be due to inhibition of PKA, actin polymerisation or CAMKII and PLD pathways, which needs to be further interrogated

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My recipe with Sterile Water: 20mL @500mg/mL 10.1g L-Carnitine Powder 12.25mL Sterile Water for Injection (W.F.I.) 25mL Benzyl Alcohol My recipe with Bacteriostatic Water: 20mL @500mg/mL 10.1g L-Carnitine Powder 12.5mL Bacteriostatic Water 1

Baig et al

After treatment, tissues were maintained in 2% FBS DMEM until the 10th days, the medium was changed every 3 days