Methods: To investigate the in vitro effects of CB-839 on CLL cells, we tested CB-839 alone and in combination with ibrutinib, venetoclax, or AZD-5991 on the HG-3 and MEC-1 CLL cell lines and on primary CLL lymphocytes
The reconstituted glutathione solution must be stored in a refrigerator at a temperature between 2C and 8C (36F and 46F)
The CellTiter-Glo 2.0 Assay uses a single, ready-to-use liquid reagent that eliminates the substrate reconstitution step required with the original two-component kit
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Notably, the contribution of the Gardos channels to eryptosis-associated cell shrinkage is supported by the fact that their inhibitors charybdotoxin and clotrimazole can avert cell shrinkage [78, 79]
See an example test result here