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The membranes were blocked using 2% BSA in Tris-buffered saline, 0.05% TWEEN (TBS-T) for 60min and incubated overnight at 4C with the indicated primary antibodies

Do not use if pregnant

Existing studies have identified several gene polymorphisms associated with osteoporosis, such as VDR gene polymorphisms, which affect individual responses to vitamin D and, consequently, bone density and health (26)

This kind of transporter system is not yet known

coli strain Nissle 1917 (EcN), and the immunomodulatory components of heat inactivated VSL#3