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The cell culture plates were examined under fluorescence microscopy to evaluate cell binding

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doi:10.1016/j.cjca.2011.07.007 Sinha A, Rahman H, Perera D

PMID 18042647

2.15 Ultrastructural analysis of SNAP-GLP-1R localization by electron microscopy INS-1 832/3-SNAP-GLP-1R cells on Thermanox coverslips (Agar Scientific) were labeled with 2 M SNAP-Surface-biotin (a gift from Dr Ivan Corra Jr, New England Biolabs), and 5 g/ml NaN 3 -free Alexa Fluor 488 Streptavidin, 10 nm colloidal gold (Molecular Probes) and stimulated with 100 nM exendin-4 for 1 hour