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Sunlight provides vitamin D but it is not safe for our skin to be exposed from it the fact that our ozone layer is much depleted and so will not protect us from UV rays

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1 2 3 4 Archives of Internal Medicine

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A less obvious B12 deficiency can also develop, marked by elevated homocysteine levels

Quantification of CLEC7A by flow cytometry Cells were adjusted to a concentration of 5 10 5 cells/ml in PBS containing 0.5% BSA and incubated with the PE-conjugated CD369 (Clec7a, Dectin-1) monoclonal antibody (Thermo Fisher Scientific, 129856-42, RRID: AB_2572749) and the corresponding IgG2a-PE isotype control (Thermo Fisher Scientific, 12-4724-82, RRID: AB_470064) (dilution 1:20) for 20 min at 4 C