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Nevertheless, some studies suggest that Ex-4 significantly stimulates hMSC proliferation in a dose- and time-dependent-manner, particularly at concentrations between 10 nM and 100 nM, as shown in cell viability assays 3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)2-(4-sulfophenyl)2 H-tetrazolium (MTS) and Cell Counting Kit-8 (CCK-8), as well as DNA analysis 5-ethynyl-2-deoxyuridin (EdU) incorporation and PicoGreen assay [14,15,16]

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Integrative Genomics Outlines a Biphasic Glucose Response and a ChREBP-RORgamma Axis Regulating Proliferation in beta Cells

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Moreover, we confirmed that the conjugate is degraded in vitro by incubation under high concentrations of glutathione (Extended Data Fig