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On day 6, cells were counted, and 100 l cell suspension (in DMEM media supplemented with only 10% FBS but no antibiotics) was added to the 96-well plates (containing siRNA and transfection mix) at the appropriate seeding density (12k for DMSO and 15k for etoposide-treated cells)

Any "fix" that ignores the acetaldehyde problem isn't a fix at allit's a risk you shouldn't take

doi: 10.1016/j.ejmech.2022.114205 6 AhmedEAOmarHMAelghaffarS Kh AbdRagbSMMNasserAY

Many studies have been carried out in the recent decades, in vivo and in vitro, as it is significant to know how the placenta functions both as a transport region and as a place of metabolism of diverse drugs and chemicals, which helped the scientific community to understand the mechanisms of transport and metabolism through the placenta, while the development of the ex vivo placental perfusion method gave us a significantly clearer picture of the toxicokinetics of the placenta [133]

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[Abstract]2025 Apr 3;15(1):11486