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The number of viable progeny and unhatched eggs was recorded

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Originally identified by Loren Pickart in human plasma, GHK-Cu has been the subject of extensive cellular biology and biochemical research literature for over five decades, with research contexts examining tissue biology, cellular signaling pathways, copper-binding peptide chemistry, and extracellular matrix research

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Given that both cysteine and GSH contribute to cell metabolism, which may be a confounding factor in MTT assay, we verified the extent of cell death by propidium iodide (PI) staining