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Quantitation was performed with 1,3,5-tribromobenzene as the internal standard and the D 1 NMR acquisition delay parameter in Topspin was set to 10 s to ensure accurate NMR integrations

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Patients should not attempt to accelerate dose increases independently, as this significantly raises the risk of adverse effects including nausea, vomiting, and gastrointestinal discomfort

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Peptides were then extracted and analyzed using a mass spectrometer

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