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At the end of treatment, mice were euthanized by asphyxiation with CO 2 and peripheral blood, spleen single cell population were analyzed for the ROS, ferroptosis levels by DCF and Bodipy-C11 staining followed by flow cytometry analysis

Introduction Reactive oxygen species (ROS) are generated by all cells during normal oxidative respiration and, if left unchecked by antioxidant systems, can cause oxidative damage to DNA, proteins, and membrane lipids

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doi: 10.3389/fmed.2023.1122529

This works best for users who already incorporate vitamin C, zinc, and antioxidants in their diet

& Pradhan, S