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Independent domains To ferret out how the mitochondria does it, the researchers used a combination of biochemical studies, computational methods, and genetic screens to discover that SLC25A39 is both a sensor and a transporter at the same time, Birsoy explains

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200 L aliquots were mixed with 400 L of a saline buffer solution (50 mM sodium phosphate buffer in D 2 O containing 0.05% of the internal standard TSP, trimethylsilyl) propionate-2,2,3,3,- d4 , for chemical shift calibration, and 4.5 mM NaN 3 , at a pH 7.4) to minimize the variation in the pH