doi: 10.7326/0003-4819-152-7-201004060-00010 5 CoffeyCMCrowsonCSMyasoedovaEMattesonELDavisJM3rd
Malondialdehyde (MDA) and Hydrogen peroxide (H 2 O 2 ) concentrations The accumulation of MDA because of lipid peroxidation was assessed by the Thiobarbituric acid (TBA) according to Wang et al
This creates a continuous, sustained elevation of GH ideal for long-term body composition studies
10.1177/1934578x1300801236 [DOI] [PubMed] [Google Scholar] Morishima C., Shuhart M
doi: 10.1016/j.cell.2016.05.041 78
Immunoblot analysis For detection of Gclc (Santa Cruz Biotechnology sc-390811), Pdha-1 (Abcam ab168379), DRP1 (Abcam ab184247), eIF4E (Abcam ab33766), p-eIF4E (Abcam ab76256), 4E-BP1 (Cell signaling 9452), p-4E-BP1 (Cell signaling 2855), DRP1 (Abcam ab184247), mTOR (Cell Signaling 2972), p-mTOR (Ser2448) (Cell Signaling 2971), p70 S6 (Cell Signaling 9202), p-p70 S6 (Thr421/Ser424) (Cell Signaling 9204), 15 10 6 cells were lysed with lysis buffer (CST 9803S) and protein/phosphatases inhibitors as per manufacturers protocol and blotted as described 44
