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doi: 10.1016/0304-3940(88)90517-4

Global analysis of the mitochondrial proteome in response to cellular GSH depletion To identify mitochondrial proteins regulated by GSH availability, we used quantitative proteomics and analyzed mitochondria immunopurified from HeLa cells expressing a mitotag (3XHA-OMP25-mCherry) grown in standard media or treated with the glutathione synthesis inhibitor, buthionine sulfoximine (BSO) 13

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Mixture will heat to about 170F

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Nuclear receptor coactivator 4 (NCOA 4) serves as a selective cargo receptor that targets ferritin to autophagosomes (Le et al., 2024)