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The PCR product was digested with Sac I and Pst I and then inserted into the sites of pTrc99a digested with the same enzymes to form the plasmid pTrc99a- as

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AR is bound to the promoter region of the RNF19A gene, regulating expression of circRNF19A and circRNF19A-490aa [141]

Some experts have raised concerns about the safety of using emerging peptides that are still considered experimental