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Quantification of the amount of VCAM-1 staining was performed with ImageJ software and plotted at the positive stain over the total tissue area
All statistical analyses were conducted using the TwoSampleMR (for extracting genetic associations where publicly available and harmonization), MendelianRandomization and MRPRESSO (for deriving MR estimates), and meta package (for assessing heterogeneity in sex differences) in R (version 4.0.1, R Foundation for Statistical Computing, Vienna, Austria)
10.1016/j.mce.2014.08.014 72 HuangL.YaoT.ChenJ.ZhangZ.YangW.GaoX.et al (2022d)
Keep vials in the original package to protect from light exposure (Light Sensitive)
Una vez preparada la solucin experimental, refrigeracin documentada cuando corresponda y registro de cada manipulacin
