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During the first three months, nausea and reduced appetite naturally limited portion sizes

B., Miller, R

Appetite changes, hormonal shifts, and new nutrient needs are all part of the process

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Western blotting Frozen cortex or hippocampal samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail

Modified tryptic peptides were identified using an Information Dependent Acquisition (IDA) LC-MS/MS workflow