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Database searching was performed in Proteome Discoverer (PD, Thermo Fisher Scientific) v3.0 against the UniProt human reference proteome, allowing tryptic peptides (730 aa), up to two missed cleavages, and up to three variable modifications per peptide

Further research is warranted to identify other risk factors that influence spleen stiffness across the spectrum of liver diseases

Changes to the production, degradation, and response pathways of these neurotransmitters may affect cellular functions including differentiation, migration, and apoptosis

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Peer review Peer review information Communications Biology thanks Chao Mao and the other, anonymous, reviewer(s) for their contribution to the peer review of this work