L-Carnitine L-Carnitine 6000mg (10ml Vial) For Research Use Only L-Carnitine is a naturally occurring compound that plays a critical role in the transportation of long-chain fatty acids into the mitochondria for energy production
Immunofluorescence and confocal microscopy Cells were labeled at 37 C with 1 M SNAP-Surface 488 in HEPES-bicarbonate buffer (120 mM NaCl, 4.8 mM KCl, 24 mM NaHCO 3 , 0.5 mM Na 2 HPO 4 , 5 mM HEPES, 2.5 mM CaCl 2 , and 1.2 mM MgCl 2 , saturated with 95% O 2 /5% CO 2 , pH 7.4) plus 3 mM glucose and 1% BSA, stimulated with agonists at 11 mM glucose for the indicated times, fixed and processed for immunofluorescence with mouse anti-Rab11 (610657, BD Biosciences, 1/20) or anti-rodent GLP-1R monoclonal antibody (Mab 7F38, DSHB, 2 g l 1 ) plus Alexa Fluor 546 secondary (Life Technologies, 1/200), mounted in Prolong Diamond antifade reagent with 4,6-diamidino-2-phenylindole (Life Technologies) and imaged with a Zeiss LSM-780 inverted confocal laser-scanning microscope in a 63/1.4 numerical aperture oil-immersion objective and analyzed in Image J
The NFS is based on age, BMI, high blood glucose level, platelet count, albumin level, and AST/ALT ratio
In this case, BMI was reported and the average BMI did increase by 0.8 BMI units in the low LH group and 0.3 units in the normal LH group
Certificates of Analysis (COAs) are available upon request to confirm purity and quality
Reconstitution Tip: Cold BAC water works
