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The original prebiotic definition of chemicals based on carbohydrates was expanded to include non-carbohydrate substances such as PUFAs, polyphenols, etc

To study the intracellular levels of ROS and GSH in viable cells, the samples were incubated with 10 M CellTracker Blue (Thermo Fisher Scientific, Barcelona, Spain) and 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 38.5 C, followed by 0.5 M PI staining

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8A) manner, at concentrations (10100 M) relevant to human disease (up to 150 M in patients with CKD) 19