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Yan X, Jiang E, Weng HR (2015) Activation of toll like receptor 4 attenuates GABA synthesis and postsynaptic GABA receptor activities in the spinal dorsal horn via releasing interleukin-1 beta
Immunoblot analysis For detection of Gclc (Santa Cruz Biotechnology sc-390811), Pdha-1 (Abcam ab168379), DRP1 (Abcam ab184247), eIF4E (Abcam ab33766), p-eIF4E (Abcam ab76256), 4E-BP1 (Cell signaling 9452), p-4E-BP1 (Cell signaling 2855), DRP1 (Abcam ab184247), mTOR (Cell Signaling 2972), p-mTOR (Ser2448) (Cell Signaling 2971), p70 S6 (Cell Signaling 9202), p-p70 S6 (Thr421/Ser424) (Cell Signaling 9204), 15 10 6 cells were lysed with lysis buffer (CST 9803S) and protein/phosphatases inhibitors as per manufacturers protocol and blotted as described 44
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For perimetry, we performed automated visual field testing (Humphrey Field Analyzer, Carl Zeiss Meditec, Jena, Germany) using 302 Swedish Interactive Threshold Algorithm (SITA) standard protocols, quantifying the mean deviation in decibels (dB) of all test locations compared to age-matched controls and defining abnormal perimetry as a mean deviation lower than -2 dB
10.1016/j.regpep.2009.05.008 3 BarisicI.BalenovicD.KlicekR.RadicB.NikitovicB.DrmicD.et al (2013)
