doi:10.1210/endo-124-4-1794
In a microwell ELISA plate pre-coated with anti-mouse glutathione peroxidase (GSH-Px) antibody (solid phase antibody), add mouse glutathione peroxidase (GSH-Px) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid surface of the microwell plate
Buckway CK, Guevara-Aguirre J, Pratt KL, Burren CP, Rosenfeld RG
As mentioned previously, although reduced glutathione, GSH, is the best form of glutathione to take, its absorption is hindered by its deactivation by stomach acids
In this regard, restoring the basement membrane and preventing the release of melanocytes and melanin into the dermis layer would be essential for long-term treatment of melasma
Lemjabbar H, Gosset P, Lechapt-Zalcman E, Franco-Montoya ML, Wallaert B, Harf A, Lafuma C