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The lowest amount tested (100 g of fresh blueberries equiv., containing 129 mg of anthocyanins) showed significant improvements in FMD, with a maximum amount equivalent to 240 g of fresh blueberries, after which the effects plateaued and didnt improve further (Reference Rodriguez-Mateos, Rendeiro and Bergillos-Meca39)

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Administering an iodide supplement between 100 and 300 g/d increased the total antioxidant status in human serum (54)

Discuss chromatographic methods you would use to purify this protein (if using ion exchange, specify the pH of the buffer)