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The V4 and V5 hypervariable regions of the bacterial 16S rRNA gene were sequenced using the Illumina MiSeq platform

miR-21: A multifaceted regulator of diabetic complications Recent studies have identified miR-21 as a key regulator of insulin/IGF signaling pathways, establishing it as a potential diagnostic marker and therapeutic target for T2DM [167]

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The living and dead G422 cells after SDT were further stained by Calcein-AM and Propidium Iodide (PI), respectively

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