The practical rule is simple
mL mg/mL Reconstituting your vial Reconstitution is adding liquid, almost always bacteriostatic water, to a freeze-dried (lyophilized) peptide so you can measure and inject it
In comparison, the pure chemical protocol from Du et al
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Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set the counter to zero with distilled Preheat Reagent II in water bath for 30 minutes: 37 (mammal cell) or 25 (other species)