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Comparing AOD 9604 and tirzepatide weight loss outcomes requires acknowledging that these compounds were tested under different conditions, for different durations, and with different outcome measures

The phosphate is what prevents NMN from entering cells directly. Conversion Pathway NR enters cells directly, where its converted to NMN by NR kinase, and then to NAD+

We included R175H, a hotspot mutant that has been previously published as sensitive, as our positive control

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NIH/3T3 cells were seeded on 6-well plates (2 10 4 cells per well) and cultured for 24 h