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Images were collected using the 365 nm (DAPI), 488 nm (Alexa-488) and 543 nm (Alexa-594) channels on a Zeiss LSM confocal microscope by sequential line scanning

For LCMS analysis, metabolite extracts were analysed by high-resolution mass spectrometry with an Orbitrap Exploris 240 (Thermo) coupled to a Vanquish Flex liquid chromatography system (Thermo)

Increased serum coagulation factors and type 1 diabetes Prothrombin (F2) was more abundant in the ND individuals relative to the UFMs, and was similarly reported in the serum proteomics study by do Nascimento de Oliveira et al [9]