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Medakas were sacrificed via overdose of anaesthesia

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Choline also influences intestinal and hepatic lipid metabolism and may contribute to maintaining normal gut function through its effects on phospholipid synthesis and membrane integrity

Trimethyl-lysine residues are the primary precursors for carnitine biosynthesis, which are hydroxylated on the third carbon by the enzyme trimethyl-lysine deoxygenase (TMLD) in mitochondria of liver, kidney, heart, muscle and brain [5]

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