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doi: 10.3389/fimmu.2023.1226443 29 OhlKTenbrockK

2023 Sep 1;41(25):4097-4106

The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

Titration is key: Gradual dose escalation maximizes tolerability

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Experimental research has explored ozone enemas in rat colonic mucosa models: Europe PMC (also available via Springer: Springer)