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Samples were thawed at room temperature and cytokine concentrations (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN, TNF, soluble Fas, soluble FasL, granzyme A, granzyme B, perforin and granulysin) was quantified using the LEGENDplex Human CD8/NK V02 Panel (BioLegend, 741187), according to the manufacturers instructions