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Acetylation involves the addition of an acetyl group (CHCO-) to the N-terminus, neutralizing the positive charge

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For both analyses, groups were first analysed using univariate logistic regression for each variable separately

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Different analyzers used in high resolution MS, including time of flight (TOF), and Orbitrap enable the determination of the elemental composition (assignment of the most probable sum formula) of a compound by measuring the exact mass and comparing the isotopic profile of the obtained spectrum with the theoretical profile

By sourcing from a supplier that independently verifies every batch, researchers can have confidence that their experimental outcomes reflect GHK-Cu activity not assay artifacts from impurities or degradation products