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The levels of telomeric DNA in samples were quantified using qPCR, the reference gene used was the single-copy gene 36B4, with primers for both telomeric and 36B4 sequences outlined in Table 1

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(PMID 12937682) provided direct laboratory evidence that Epithalon induces telomerase activity and telomere elongation in human somatic cells the foundational finding supporting its anti-aging applications

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