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Journal of Applied Polymer Science, 134 , 44498

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Then, 2 mL of sperm preparation medium was added to the tube again and centrifuged at 328 g for 5 min, and then the supernatant was again removed

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Then, cells were treated with either vehicle (DMSO) or AG-120 (1 M) for 6-8 hours under indicated Mg 2+ levels