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This step might involve acid-base catalysis by the active-site cysteinyl thiolate (which could explain an effect of the second cysteinyl residue), and/or the conserved lysine residue, yielding GS in analogy to glutathione transferases (Fig

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[DOI] [PubMed] [Google Scholar] Hseu YC, Vudhya Gowrisankar Y, Chen XZ, Yang YC, Yang HL