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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)

GGT is checked for several reasons, though many clinicians underutilize it

People bring it up for issues like gut irritation, leaky gut conversations, or inflammatory bowel related models in research contexts

Roughly 90 per cent of drugs that show promise in animals ultimately fail in human trials

The trajectory is flat or downward rather than upward