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To assess if the metabolism of TNBS by NAPDH-linked enzymes in aerobic solution results in formation of ROS, we determined the formation of hydrogen peroxide in an aqueous solution (PBS) containing 0.25 mm NADPH, TNBS (1 mm) and GSSG reductase (15 U/ml) over time

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Therefore, maintaining its integrity is a non-negotiable aspect of laboratory operations